These pre-poured agar slants are for long-term storage of mushroom cultures. Each slant contains approximately 10 ml of pre-sterilized potato dextrose agar (PDA) in a 15 ml polypropylene tube. To use: Allow your agar slant to equilibrate at room temperature before use. Then, in a sterile environment, transfer mycelium from a plate into slant. Store in the refrigerator for up to a year.  Storing unused slants: Store your unused slants upright in a cool location. In the absence of a refrigerator, store them in a cold room.  Check for condensation from time to time. Condensation means there is a heat source driving out water from the agar, which will dry it out quickly. Use within 1 year of receipt.