Overview Methylene Blue — 500 mg (Liquid) from Peptide Tech is a premium, ready-to-use research-use-only (RUO) aqueous solution of methylthioninium chloride, supplied with batch traceability and QR-linked documentation. This lab dye and redox indicator is suitable for in-vitro staining, photochemistry, electron-transfer experiments, and analytical method development where identity, stability, and light-protection matter. Specifications Property Details Chemical Name Methylthioninium chloride (Methylene Blue) Synonyms Basic Blue 9; Tetramethylthionine chloride; Methylene Blue CAS Number 61-73-4 PubChem CID 6099 Molecular Formula C16H18ClN3S Molecular Weight ≈ 319.85 g/mol Form Ready-to-use aqueous solution (liquid) Net Content 500 mg active dye per bottle (concentration/volume printed on label & COA) Appearance Deep blue solution; filled in light-protective (amber) container Purity / ID Identity confirmed by MS/UV-Vis; third-party verified. See lot COA via on-vial QR. Sterility 0.22 µm sterile-filtered and aseptically filled (see COA for lot-specific confirmation) Solubility Water-miscible; compatible with many neutral aqueous buffers. Protect from strong light; avoid oxidizing/strong reducing agents unless intended for the experiment. Intended Use Laboratory research only (RUO). Not for human or animal use. Molecular Structure View on PubChem (CID 6099) Key Features of Peptide Tech Same-Day Shipping (Mon–Fri, on in-stock items) GMP-Aligned production & documentation USA Made 24/7 Support Price Match Guarantee Batch Tracking with QR Code Verification Third-Party U.S. Lab Testing — identity, quantity, endotoxin (where applicable) Cold-Chain Shipping — Always (no paid upgrade) Worldwide Shipping All Major Payments Accepted Text & Email Order Updates 2-Day FedEx/UPS Standard with low-cost overnight Largest Variety of Peptides & Bioregulators Potential Applications in Research Strictly in-vitro or analytical contexts. No health, diagnostic, or therapeutic claims. Redox & photochemistry — electron-transfer studies, photosensitization, and light-driven reactions with controlled illumination. UV-Vis method development — dye calibration, extinction-based quantitation, and instrument performance checks. Cell-free/microbiology staining — model staining and visualization protocols under controlled conditions. Assay QC/controls — colorimetric assay benchmarking and stability checks in buffered matrices. Chromatography/LC–MS sample prep — test analyte for method validation (carryover, recovery, light-stress studies). Quality, Storage & Handling Every lot is supported by a QR-linked COA. Independent U.S. labs verify identity and quantity, with endotoxin testing where applicable. Storage: 2–8 °C; protect from light; keep tightly closed. Do not autoclave the filled container. Handling: Use light-minimizing practices; avoid metal contamination; dye will permanently stain porous surfaces and some plastics. Shelf life: See lot COA and label. Use clean, dedicated tools to avoid introducing reductants/oxidants. Shipping & Fulfillment Ships in validated cold-chain packaging. In-stock orders placed before the daily cutoff ship the same day. 2-Day FedEx/UPS is standard, with overnight options available. Automated text/email updates included; international shipping available where permitted. Compliance & RUO Disclaimer For laboratory research only. Not a drug, cosmetic, food, or household chemical. Not for human or animal use, ingestion, diagnostic, or therapeutic applications. Buyers warrant they are qualified to handle RUO materials under applicable laws and institutional guidelines. FAQs What is the concentration and volume? The bottle contains 500 mg of active dye in water. The exact concentration (mg/mL) and fill volume are printed on the label and listed on the COA (scan the on-vial QR). Is it sterile? Lots are 0.22 µm sterile-filtered and aseptically filled unless otherwise noted. Confirm sterility status on the COA for your lot. How should I store and handle it? Store at 2–8 °C, protected from light. Use amberware when possible, minimize light exposure during experiments, and avoid introducing strong oxidants/reductants unless required by your protocol. Can I use this in living systems? No. This product is sold strictly as RUO and is not for human or animal use. Explore More Browse additional research reagents and accessories at Peptide Tech.