Human Latency-Associated Peptide (TGFbeta1-LAP) ELISA Kit

This Human Latency-Associated Peptide (TGFbeta1-LAP) Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of human LAP(TGF-beta1) in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests. This human LAP(TGF-beta1) Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LAP(TGF-beta1) has been precoated onto 96-well plates. Standards(sf21, L30-R278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LAP(TGF-beta1) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LAP(TGF-beta1) amount of sample captured in plate. Detection Target: Latency-Associated Peptide (TGFbeta1-LAP) Uniprot ID: P01137) Reactivity: Human Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 62.5pg/ml-4000pg/ml Sensitivity: <10pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice) Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.; Immunogen sequence: 369 Click here to view a video tutorial covering frequently asked questions regarding ELISA workflows.